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goat anti human dkk 3  (R&D Systems)


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    R&D Systems goat anti human dkk 3
    Goat Anti Human Dkk 3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dkk+3+antibody/Human+Dkk-3+Antibody/10__7554_slash_elife__89453__3-445-32-38
    Average 93 stars, based on 28 article reviews
    goat anti human dkk 3 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: Elevated levels of Dickkopf-related protein 3 in seminal plasma of prostate cancer patients
    Article Snippet: .. A series of six monoclonal Antibodies (mAb) INN(sbruck)-Dkk3-1 to INN-Dkk3-6 as previously described [ ] and an affinity purified polyclonal goat anti Dkk-3 antibody (R&D Systems Cat.#AF1118) were characterized by radioimmunoassays (RIA) according to affinity, molecular epitope localization and specificity with native (untagged) Dkk-3 and the commercially available homologous proteins Dkk-1, Dkk-4 and Sgy-1 (R&D Systems) as described elsewhere [ ]. ..

    Affinity Purification:

    Article Title: Elevated levels of Dickkopf-related protein 3 in seminal plasma of prostate cancer patients
    Article Snippet: .. A series of six monoclonal Antibodies (mAb) INN(sbruck)-Dkk3-1 to INN-Dkk3-6 as previously described [ ] and an affinity purified polyclonal goat anti Dkk-3 antibody (R&D Systems Cat.#AF1118) were characterized by radioimmunoassays (RIA) according to affinity, molecular epitope localization and specificity with native (untagged) Dkk-3 and the commercially available homologous proteins Dkk-1, Dkk-4 and Sgy-1 (R&D Systems) as described elsewhere [ ]. ..

    Blocking Assay:

    Article Title: Increase of Dkk-3 blood plasma levels in the elderly.
    Article Snippet: 0531-5565/$ see front matter 2008 Elsevier Inc. A doi:10.1016/j.exger.2008.05.012 Abbreviations: BPH, benign prostatic hyperplasia; noenzymometric assay; FSH, follicle stimulating h peroxidase; LH, luteinizing hormone; mAb, monoclona binding; PCa, prostate carcinoma; SAGE, serial analys * Corresponding author.. Tel.. : +43 512 583919 24; f E-mail address: peter.berger@oeaw.ac.at (P. Berger Gene expression of the secreted glycoprotein Dkk-3 is upregulated during cellular senescence in prostate basal epithelial cells and altered in age-related disorders of the human prostate.

    Incubation:

    Article Title: Increase of Dkk-3 blood plasma levels in the elderly.
    Article Snippet: 0531-5565/$ see front matter 2008 Elsevier Inc. A doi:10.1016/j.exger.2008.05.012 Abbreviations: BPH, benign prostatic hyperplasia; noenzymometric assay; FSH, follicle stimulating h peroxidase; LH, luteinizing hormone; mAb, monoclona binding; PCa, prostate carcinoma; SAGE, serial analys * Corresponding author.. Tel.. : +43 512 583919 24; f E-mail address: peter.berger@oeaw.ac.at (P. Berger Gene expression of the secreted glycoprotein Dkk-3 is upregulated during cellular senescence in prostate basal epithelial cells and altered in age-related disorders of the human prostate.

    Concentration Assay:

    Article Title: Increase of Dkk-3 blood plasma levels in the elderly.
    Article Snippet: 0531-5565/$ see front matter 2008 Elsevier Inc. A doi:10.1016/j.exger.2008.05.012 Abbreviations: BPH, benign prostatic hyperplasia; noenzymometric assay; FSH, follicle stimulating h peroxidase; LH, luteinizing hormone; mAb, monoclona binding; PCa, prostate carcinoma; SAGE, serial analys * Corresponding author.. Tel.. : +43 512 583919 24; f E-mail address: peter.berger@oeaw.ac.at (P. Berger Gene expression of the secreted glycoprotein Dkk-3 is upregulated during cellular senescence in prostate basal epithelial cells and altered in age-related disorders of the human prostate.

    Expressing:

    Article Title: Long-Lasting Molecular Changes in Human Skin after Repetitive in situ UV Irradiation
    Article Snippet: .. Protein expression using the following antibodies was measured: SCF antibody (at 1:10 dilution; R&D Systems, Minneapolis, MN, USA), SCF R/KIT antibody (at 1:100 dilution; R&D Systems), bFGF antibody (at 1:10 dilution; R&D Systems), FGFR1 antibody (at 1:100 dilution; Abcam Inc, Cambridge, MA, USA), ET-1 antibody (at 1:200 dilution; Abcam Inc), EDNRB antibody (at 1:100 dilution; Abcam Inc), GM-CSF antibody (at 1:20 dilution; R&D Systems), HGF antibody (at 1:10 dilution; R&D Systems), PCNA antibody (at 1:200 dilution; Dako Inc, Carpinteria, CA, USA), cyclin D1 antibody (at 1:20 dilution; Zymed Laboratories, South San Francisco, CA, USA), cyclin E2 antibody (at 1:20 dilution; Cell Signaling Technology, Beverly, MA, USA), E-cadherin antibody (at 1:100 dilution; Takara Bio Inc, Shiga, Japan), N-cadherin antibody (at 1:10 dilution; Dako Inc), β-catenin antibody (at 1:100 dilution; Cell Signaling Technology), Bcl-2 antibody (at 1:20 dilution; Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA), DKK-1 antibody (at 1:50 dilution; R&D Systems) and DKK-3 antibody (at 1: 100 dilution; R&D Systems). ..

    Labeling:

    Article Title: Elevated levels of Dickkopf-related protein 3 in seminal plasma of prostate cancer patients
    Article Snippet: .. To increase sensitivity and to allow higher sample throughput, the assay was commuted to a 96-well plate based indirect immunoenzymometric assay (IEMA) described previously [ ] by replacement of the 125 l labeled antibody by a biotinylated variant of the polyclonal goat anti Dkk-3 antibody (R&D Systems Cat.#BAF1118). ..

    Variant Assay:

    Article Title: Elevated levels of Dickkopf-related protein 3 in seminal plasma of prostate cancer patients
    Article Snippet: .. To increase sensitivity and to allow higher sample throughput, the assay was commuted to a 96-well plate based indirect immunoenzymometric assay (IEMA) described previously [ ] by replacement of the 125 l labeled antibody by a biotinylated variant of the polyclonal goat anti Dkk-3 antibody (R&D Systems Cat.#BAF1118). ..



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    Image Search Results


    Journal: eLife

    Article Title: Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model

    doi: 10.7554/eLife.89453

    Figure Lengend Snippet:

    Article Snippet: Primary antibodies and dilutions used for western blot were: Mouse Anti- Aβ (6E10 clone, 1:1000, Biolegend, Cat# 803001, RRID: AB_2564653 ), goat anti- Mouse DKK-3 (1:1000, R and D Systems, Cat# AF948, RRID: AB_355734 ), goat anti- Human DKK-3 (1:1000, R&D Systems, Cat# AF1118, RRID: AB_354610 ), rabbit anti-GAPDH (1:5000, Abcam, Cat# ab181602, RRID: AB_2630358 ), rabbit anti-GluA1 (1:1000, Cell Signaling Technology, Cat# 13185, RRID: AB_2732897 ), rabbit anti-phospho GluA1 Serine 845 (1:1000, Cell Signaling Technology, Cat# 8084, RRID: AB_10860773 ), rabbit anti- SAPK/JNK (1:1,000, Cell Signaling Technology, Cat# 9252, RRID: AB_2250373 ), mouse anti-Phospho-SAPK/JNK (Thr183/Tyr185) (1:500, Cell Signaling Technology, Cat# 9255, RRID: AB_2307321 ), mouse anti-Tubulin (1:5000, Sigma-Aldrich, Cat# T9026, RRID: AB_477593 ), mouse Anti-Vinculin (1:2000, Sigma Aldrich, Cat# v4505, RRID: AB_477617 ), HRP mouse anti-beta Actin (1:10,000, Abcam, Cat# ab8224, RRID: AB_449644 ).

    Techniques: Isolation, Knock-Out, Control, Sequencing, shRNA, Recombinant, TUNEL Assay, cDNA Synthesis, Fluorsave, Software, Microscopy, Immunofluorescence, Patch Clamp